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KMID : 0370219970410010074
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Yakhak Hoeji
1997 Volume.41 No. 1 p.74 ~ p.80
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Quantitation of Recombinant Hirudin by Enzyme-Linked Immunosorbent Assay
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최윤주/Choi YJ
한범수/안미영/박병근/손정훈/최의성/이상기/김영식/Hahn BS/Ahn MY/Park PK/Shon JH/Choi ES/Lee SK/Kim YS
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Abstract
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A polygonal antibody against recombinant hirudin was raised for the development of a ELISA in biological fluids. Recombinant hirudin was conjugated to maleimide activated carrier protein, KLH and injected to a rabbit. The third booster collection of antiserum was used as primary antibody for the ELISA. The titer for the detection antibody was determined. The direct ELISA could determine the concentration of hirudin in the range of ~10ng/ml. Affinity pulified IgG was obtained and conjugated to horseradish peroxidase. Purified IgG and IgG-HRP could be used as capture and detection antibody, respectively. Although sandwich ELISA would not give the satisfactory results. it could apply for the detection of hirudin level in the range of ~20mcg/ml.
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